Dietary High Glycinin Reduces Growth Performance and Impairs Liver and Intestinal Health Status of Orange-Spotted Grouper (Epinephelus coioides)

The aim of the study was to investigate whether negative effects dietary glycinin are linked structural integrity damage, apoptosis promotion and microbiota alteration in intestine orange-spotted grouper (Epinephelus coioides). basal diet (FM diet) formulated contain 48% protein 11% lipid. Fish meal replaced by soybean (SBM) FM diets prepare SBM diet. Two experimental were prepared, containing 4.5% 10% (G-4.5 G-10, respectively). Triplicate groups 20 fish each tank (initial weight: 8.01 ± 0.10 g) fed four across an 8 week growth trial period. had reduced rate, hepatosomatic index, liver total antioxidant capacity GSH-Px activity, but elevated MDA content vs. diets. G-4.5 exhibited maximum G-10 a comparable with that group. down-regulated intestinal tight junction function genes (occludin, claudin-3 ZO-1) (caspase-3, caspase-8, caspase-9, bcl-2 bcl-xL), blood diamine oxidase D-lactic acid endotoxin contents related mucosal permeability, as well number vs abundance phylum Proteobacteria genus Vibrio higher than those receiving other As for expression inflammatory factor genes, diets, pro-inflammatory (TNF-α, IL-1β IL-8) up-regulated, anti-inflammatory (TGF-β1 IL-10) down-regulated. results indicate rather could decrease hepatic ability destroy both profile morphological through disrupting structure intestine, increasing permeability apoptosis. These further trigger reactions even enteritis, ultimately leading poor fish.